The molarity of HCl is 1.0 M. the volume of HCl is calculated as follows:
The volume of HCl = Number of moles/Molarity
0.017 mol/1.0 mol/L
= 0.017 L
=17 mL
Since the total volume of the buffer solution is 1 L. The volume of water is calculated as:
The volume of water = 1 - Volume of HCI
= 1-0.017
= 0.983 L
Hence, to prepare a phosphate buffer, 7.1 g of Na, HPO4, 17 mL of HCl, and 0.983 L of water are mixed together.
Phosphate-buffered saline is a commonly used buffer in biological research. This is an aqueous saline solution containing disodium hydrogen phosphate, sodium chloride, and in some formulations potassium chloride and potassium dihydrogen phosphate. Buffers help maintain a constant pH.
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