To accomplish the dilution the microbiologist should dilute 1 mL of the bacterial culture in 100 mL of the total volume. Thus, option C is correct.
What is dilution?
Dilution is a process of decreasing the solute concentration by the addition of the solvent in the solution. The volume for the dilution is calculated as:
Given,
C1 = 100,000 cell per mL
C2 = 1000 cell per mL
V1 = 1 mL
C1 V1 = C2 V2
100,000 × (1 ÷ 1000)
= 100 mL
Therefore, 100 mL should be used to dilute 1 mL.
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