In the "pulse-chase" experiment, Palade and colleagues studied the pathway taken by newly secreted proteins in pancreatic acinar cells by labeling them with radioactive amino acids, removing unused label, and determining their location within the cells at various time points. Which sequence represents the correct order in which the proteins were identified?
A. Secretory vesicles → Golgi apparatus → rough ER → nucleus
B. Golgi apparatus → rough ER → nucleus → secretory vesicles
C. Rough ER → Golgi apparatus → secretory vesicles
D. Rough ER → secretory vesicles → Golgi apparatus

These experiments defined a pathway taken by secreted proteins, the secretory pathway: rough ER → Golgi → secretory vesicles → cell exterior.
Proteins destined for secretion or incorporation into the ER, Golgi apparatus, lysosomes, or plasma membrane are initially targeted to the ER.

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shrutiagrawal1798 shrutiagrawal1798 · Answer 2 · 2022-03-01T12:02:02+01:00

Protein synthesis in the cell takes place in the ribosomes. The protein synthesized are secreted as Rough ER followed by Golgi apparatus and subsequently to the secretary vesicles.

How proteins are synthesized?

The proteins are the building blocks of the human body. They are the chain of amino acids, and are formed in the ribosomes.

The protein synthesized in the ribosomes undergoes post translational modification, before secreting from the cell.

The modification takes place in the ER and the Golgi apparatus that assign the proteins in the vesicles for the targeted transport.

The pathway followed by the protein for their secretion out of the cell has been rough ER, Golgi apparatus, and secretory vesicles.

Learn more about protein modifications, here:

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