Given a template strand of 3'-atgcttggaca-5' and a partially-made complementary strand containing only 5'-tac-3', what would be the sequence of the new strand of dna (including the 5'-tac-3') if the only additional nucleotides available to dna polymerase were those containing the bases g, a, and c?

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PDAWGINTHEHOUSE8280 PDAWGINTHEHOUSE8280

12-11-2017
Biology

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Given a template strand of 3'-atgcttggaca-5' and a partially-made complementary strand containing only 5'-tac-3', what would be the sequence of the new strand of dna (including the 5'-tac-3') if the only additional nucleotides available to dna polymerase were those containing the bases g, a, and c?

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davidsilva2019 davidsilva2019 · Answer 1 · 2017-11-22T13:59:06+01:00

A complementary strand is complementary to the template strand when in the opposite direction, in this case 5'-3', the nucleotides that it contains are complementary to those of the template strand. Thus, the partially-made complementary strand 5'-TAC-3' will bind to the template strand starting on the 3'-ATG-5' portion. Then it is elongated by the DNA polymerase only up to the next nucleotide A, not included, because its complementary nucleotide is T which is not available. So, the sequence of the new strand on DNA would be 5'-TACGAACC-3'.