Respuesta :
The main purpose of spreading pGLO on the LB plate is to help bacteria to grow, otherwise the ampicillin would kill them. In case ,pGLO is not spread, then the cells would be killed by the ampicillin.
The Green Fluorescent Protein takes around 20 minutes to be transcribed and translated , on the other hand Arabinose promoter needs at least 20 minutes before RNA polymerase can bind to the promoter .
In general they have phenotypic lag of 20 minutes before the b-lactamase gene for Amp resistance can be expressed . we observe no growth of bacteria because it was not exposed to pGLO plasmid and lacked ampicillin resistance. If They were exposed to AMP and arabinose sugar, this cause bacteria to grow with ampicillin resistance.
therefore, The purpose of the cells grown on the LB plate is to help bacteria to grow, otherwise the ampicillin would kill them. In case ,pGLO is not spread, then the cells would be killed by the ampicillin.
To learn more about ampicillin resistance ,here
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