The mrna is at the third position (3’ letter) wobble base of the triplet codon.
- The third nucleotide in a codon is referred to as the wobble position. There are two main properties of this nucleotide:
- The cognate tRNA's binding to a codon in an mRNA is substantially more "looser" in the codon's third position.
- The third codon position can now accommodate a variety of non-Watson-Crick base pairings.
- The 5' end of the codon is where the tRNA anticodon and mRNA codon are paired.
- The precise base pairing of the third place is less important once the first two positions have been paired.
- It "wobbles" because the third (5') base of the anticodon can often mate with either the purine or pyrimidine pair in the codon, depending on the situation.
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