The sample size required is a minimum of 30 participants.
Whenever the study sample size is very restricted (<30 measurements), the analysis of subgroups is difficult and the performance of statistical tests is compromised. However, additional care must be taken with sample oversizing, which commonly occurs when accessing large computerized databases. The increase in the sample reduces the confidence intervals of the estimates and allows the detection of differences between subgroups that, despite being statistically significant, are not clinically relevant.
With this information, we can conclude that The sample size required is a minimum of 30 participants.
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