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In the sanger (dideoxy) method for dna sequencing, a small amount of a dideoxynucle- otide triphosphate—say, ddctp—is added to the sequencing reaction along with a larger amount of the corresponding dctp. what result would be observed if the dctp were omitted?

Respuesta :

Photon
When the first G residue is encounteredin the template, ddCTP is added and polymerization halts. Only one band will appear in thesequencing gel.

Hope this helps !

Photon

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