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Answer is: Louis Pasteur (1822-1895) disproved spontaneous generation. He boiled liquid with proteins and other nutrients in S-neck flasks and regular flask to kill all microorganism present. In regular flask liquid showed changes when exposed to air and in S-neck flack there was no change (microorganism can not getting in the flask because the S- neck). When he removed S-neck, liquid changed because microorganisms came in liquid.
Pasteur showed it was not the air itself but particles within the air that had microorganisms.
Pasteur showed it was not the air itself but particles within the air that had microorganisms.
The end of the revolutionary period of the theory of abiogenesis and biogenesis was after the appearance of Louis Pasteur (1822-1895), a chemist who was very interested in chemical changes that occurred during the fermentation process of wine.
Further Explanation
Around 1860, Louis Pasteur (1822-1895) faced a challenge that would make a big difference in the ongoing controversy over the Abigenesis theory. Member of the French Academy of Sciences, F.A. Pouchet, has published the results of his experiments which he said were able to create living things spontaneously. Pasteur pointed out some weaknesses in the experimental method, while Pouchet challenged the French Academy to offer prizes to anyone who could prove Abiogenesis's theory true or false.
Pasteur conducted an experiment which was a refinement of the Lazzaro Spallanzani experiment. Pasteur uses a neck tube like a swan.
The reason is that dust particles that contain microorganisms do not reach the nutritional content, they settle in the part of the swan neck tube that forms U ablution and the airflow is reduced, so the particles are not carried into the pumpkin. If the pumpkin containing nutrients is then stored placed, tilted, so that particles can be released from the laboratory, then after a while is found microorganisms found in the pumpkin.
The experiments are as follows:
- Gray neck like a swan filled with broth. The neck of the nation was made to maintain the relationship between ash and the outside air. Then the pumpkin is heated to sterilize the broth from microorganisms.
- After that, the pumpkin is cooled and placed in a safe place. Air from outside can enter the pumpkin. Because the shape of the pipe such as the swan's neck, dust, and microorganisms in the air stick to the bottom of the swan's neck, so the air that enters the pumpkin is sterile air. So in Louis Pasteur's experimental flask, there is still a life force as questioned by followers of the Abiogenesis theory. After being left for a few days, the broth remains clear and does not contain microorganisms.
- The gray crystal-filled broth water was broken in the neck, so the broth water came into direct contact with outside air. After a few days left, the broth in the pumpkin becomes rotten and contains many microorganisms.
The conclusion of Louis Pasteur's experiment was that microorganisms in broth were not from liquid (non-living things), but from microorganisms in the air. The microorganisms in the air enter the pumpkin together with dust.
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Louis Pasteur's experiment https://brainly.com/question/8181234
Details
Grade: Middle School
Subject: Chemistry
keywords: Louis Pasteur, experiment.