The data below were collected for an enzyme-catalyzed reaction. determine the vmax and km for this reaction

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13-09-2017
Chemistry

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The data below were collected for an enzyme-catalyzed reaction. determine the vmax and km for this reaction

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meerkat18 meerkat18 · Answer 1 · 2017-09-25T16:35:05+02:00

The parameters vmax and km are determined using known models in enzyme reactions. One linear model in enzyme reactions is the Lineweaver-Burke model. Its form is written as

1/v = (Km/vmax)(1/S) + 1/vmax,
where
Km/vmax is the slope
1/vmax is the y-intercept

In order to determine the parameters, plot (1/v) vs (1/S) data, where v is the rate of reaction and S is the substrate concentration. Then, find the y-intercept which is the point at which the line intersects the y-axis. The reciprocal of the y-intercept is equivalent to vmax. For Km, find the slope through linear regression. Then, multiple vmax to the slope to determine Km.