Following are requirements for transcription initiation in prokaryotic and eukaryotic cells
1. The DNA double helix must be accessible to RNA polymerase.
2. The promoter must be able to be bound by the RNA polymerase.
A DNA sequence upstream of a gene must be bound by an RNA polymerase in order to start transcription in eukaryotic cells, just like in prokaryotic cells.
In eukaryotes, the nucleus is the starting point for transcription. Translation begins as soon as the mRNA leaves the nucleus and moves to the cytoplasm. The cytoplasm is where prokaryotes start the transcription process. Prokaryotes are exempt from the need for RNA processing.
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