contestada

Problem 7: In a translational reporter system, the gene of interest is left intact, and the reporter is fused to the end of the sequence. Which of the following is necessary to ensure the reporter expresses properly? O Removal of the stop codon in the gene of interest before the reporter sequence and the reporter gene must be in the same reading frame as the gene of interest. O A separate promoter for the reporter is required. O The reporter gene must be in the same reading frame as the gene of interest. O Removal of the stop codon in the gene of interest before the reporter sequence. None of these

Respuesta :

The use of a translational reporter system is a powerful tool for studying gene expression, as it allows researchers to track the expression of a gene of interest without altering the gene itself. However, in order for the reporter to express properly, certain measures must be taken.

The most important requirement for successful expression of a reporter in a translational reporter system is that the gene of interest must have its stop codon removed before the reporter sequence is added. This ensures that the gene of interest and the reporter gene are both in the same reading frame, allowing the reporter to be expressed properly. Additionally, it is important that the reporter is not placed in a separate promoter, as this could lead to incorrect expression levels of the gene of interest.

Learn more about stop codon at :https://brainly.com/question/13792686

#SPJ4

Otras preguntas