Respuesta :
Through transformation, the recombinant plasmid is inserted into E. coli cells. Cells are coaxed into taking up plasmid by treating the cells with Calcium Chloride followed by brief exposure to elevated temperature.
Give a brief account of E. coli.
Escherichia coli, also known as E. coli, is a rod-shaped, facultatively anaerobic, Gram-negative coliform bacteria found in the lower intestine of warm-blooded creatures. The majority of E. coli strains are not harmful, but some serotypes have the potential to seriously injure their hosts and occasionally result in food contamination episodes that force manufacturers to recall their products.
By creating vitamin K2 and inhibiting the colonization of the intestine with pathogenic bacteria, the benign strains, which are a typical component of the gut microbiota, can benefit their hosts. Within fecal matter, E. coli gets released into the environment. For three days, the bacterium grows rapidly in fresh feces under aerobic conditions, but after that, it slowly starts to decline in numbers.
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