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what will happen to the e. coli cells you spread on the lb/ampicillin plates that did not contain a pclone plasmid? what experiment could you do to test your hypothesis?

Respuesta :

The e.coli cells spreaded on the ampicilin plates without a pclone plasmid would not grow on plates.

What is a plasmid and what could be the experiment to test the hypothesis of e coli cells spreaded on the plate?

  1. Plasmid is a genetic structure of the cell which can replicate independently with the help of chromosomes present inside the cell.
  2. Here the question is asked of the e.coli cells spreaded on the ampicillin plate that is not containing a pclone plasmid.
  3. Whenever we arrange the e.coli cells on the ampicillin plates for the growth only the transformants grow and the non transformants do not grow.
  4. Here the e.coli cells is not containing the pclone plasmid that means if it would be plated on the ampicillin plate it won't grow.
  5. The experiment to test this hypothesis is plating the e.coli cells on the culture plate containing the ampicillin medium and testing it for the growth of transformants.
  6. Plasmid DNA is completely different from chromosomal DNA in the sense that plasmid DNA is extra, cellular and small doubled stranded DNA.

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