You are studying a drug that blocks translation in bacteria. You want to know more about its mechanism of action. You treat bacteria with the drug and isolate mrnas with their associated proteins from the treated bacteria. In bacteria treated with the drug, you find that small ribosomal subunits are bound to the mrnas, but not the large subunits. In which stage does this drug arrest translation?.

Respuesta :

According to the study, the drug arrest translation of proteins in the initiation stage.

Translation is the process of formation of proteins from the mRNA which is synthesized by the DNA in the nucleus of cell. Here, the drug which blocks translation in bacteria and the small ribosomal subunit (30S) binds to the mRNA but not the large ribosomal subunit (50S). So we can say that large ribosomal subunit binds to mRNA after the formation of the 30S initial complex. However, if it was treated with the drugs such as (clindamycin, macrolides, etc) in the translation process then this drug bind to the large ribosomal subunit (50S) and blocks the peptide bond formation between the amino acid and thus stop the initiation step of the translation.

So, we can say that if we use the drug in the bacterial translation process then the drug bind to the large ribosomal subunit and stop the initiation step of the translation.

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