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The technique to show that the gene is no longer expressed is DNA microarray.
The method used to determine whether a gene is expressive or not is PCR. However, the DNA microarray is a novel structure that can simultaneously measure the expression of thousands of genes.
A collection of microscopic DNA spots attached to a solid surface is referred to as a DNA microarray (also known as a DNA chip or a biochip). DNA microarrays are used by researchers to genotype multiple regions of a genome or measure the expression levels of a large number of genes simultaneously. This would help in demonstrating if the homozygous knockout mouse no longer expresses the gene. The term "probes," also known as "reporters," or "oligos," refers to the 10-12 moles of a particular DNA sequence found in each DNA spot. These can be a brief segment of a gene or another DNA element that is used to hybridize a cDNA or cRNA sample (also known as the target) under stringent conditions.
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