Respuesta :
In this experiment, increasing the concentration of lactose beyond 500mg/dl does not increase the rate of reaction further because there are no free enzymes available to react with the lactose.
Initially, when the substrate concentration is increased, the rate of reaction also increases considerably and leads to an increase in the rate of an enzyme catalysed reaction. Now as the concentration of the substrate increases, there are no free enzymes available to react with these substrate. All the active sites of all the enzymes available are already occupied by the substrate molecules and further increasing the substrate molecules beyond this point lead to no additional effect as there are no free enzymes molecules available to carry out the reaction. Hence, increasing the concentration of lactose molecule does not increase the rate of reaction further. But if we add more lactose enzymes then the rate of the reaction will increase by decreasing the activation energy.
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