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The result of the Khorana group's synthesized mRNA with repeating UCA shows the genetic code is a triplet code.
A triplet base code makes up the genetic code for life. It is well known that codon pair biases occur frequently in nature and that adjacent codons might affect the translation of a certain codon. We demonstrate that the two codons before a specific codon can influence mRNA translation at that codon. The information provided here supports a scenario in which a single codon is subject to evolutionary selection pressure over five subsequent codons, including synonymous codons. This work lays the groundwork for understanding how single DNA base changes might impact translation over multiple codons and ought to be taken into account when characterizing how DNA base changes affect human disease.
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The genetic code is a triplet made up of three codons
Khorana develop a method for in vitro RNA synthesis with defined combinati
-on of bases. He develop co-polymers
and Homopolymers
Later on he obtained polypeptide from this copolymer in which two types of
Amino acids cysteine and valine were
arranged alternately
UGU GUG UGU G - Copolymer
| | |
Cys val cys
Cys = cysteine
Val = valine
Khorana applied his knowledge of chemistry to molecular biology, which greatly aided him in deciphering the genetic code. The technology Khorana used was the chemical synthesis of nucleic acid sequences. For the experiments, he created short repeating sequences that could then be tested against specific amino acids.
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