you were provided with two different ssdna oligonucleotides from which you produced dsdna. what procedure did you perform that facilitated the assembly of dsdna? what properties of dna made this possible?

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15-11-2022
Biology

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you were provided with two different ssdna oligonucleotides from which you produced dsdna. what procedure did you perform that facilitated the assembly of dsdna? what properties of dna made this possible?

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tutorconsortium272 tutorconsortium272 · Answer 1 · 2022-11-24T10:10:29+01:00

Procedure performed that facilitated the assembly of dsDNA is : Golden Gate Assembly (GGA).

What is Golden Gate Assembly?

Golden Gate assembly is also known as Golden Gate cloning and is created by Carola Engler and colleagues in 2008.

Golden Gate Assembly is an efficient way to quickly assemble multiple DNA sequences or parts into a single plasmid.

Golden Gate assembly is a molecular cloning method that allows a researcher to simultaneously assemble multiple DNA fragments into a single piece using Type IIS restriction enzymes and T4 DNA ligase.

Golden Gate assembly utilizes a Type IIS restriction enzyme which cleaves outside of non-palindromic recognition sequence and T4 DNA Ligase in a single-tube reaction.

To know more about dsDNA, refer

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