- Some constitutive mutations to the lacI repressor were found to alter the conformation of its binding site for the operator.
- These lacF mutations produced proteins that did not repress the lac operon even when the allolactose inducer was absent.
- Another type of lacI mutant was found in which the lac operon remained suppressed even when the allolactose inducer was present.
- These mutations were termed lac[tex]I^{s}[/tex] mutants.
- Super-repressor mutants were found to alter the conformation of the repressor binding site for the inducer such that the repressor could continue to bind to the operator.
- If lactose was the only carbon source made available to an E. coli bacterium, a super-repressor mutation in lacI would be lethal.
In constitutive mutations, the defected gene product is continuously formed from the defected gene instead of the wild type. There remains no control over the expression of gene.
Lac operon is an aggregate of multiple genes all controlled by a single promoter that work in the metabolism of lactose in the body. There are three genes in lac operon: gene z , gene y and gene a.
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