The Northern blot technique uses DNA, antibody, RNA, probes, and proteins to identify biological molecules.
RNA molecules can be identified and measured via northern blot analysis. Northern blot analysis, in contrast to techniques like reverse transcriptase PCR (RT-PCR) or RNase protective assay, may identify RNA molecules by their size.
The fundamental idea behind Northern blotting is the separation of RNA into different sizes, which is then detected on a membranes using a hybridized probe with a template strand complementary to all or a portion of the sequences of the target mRNA. RNA, DNA, or oligodeoxynucleotide probes that are radioactively or non-radioactively tagged can be used to examine Northern blots.
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