Characteristic best distinguishes from prokaryotic to eukaryotic is that eukaryotic cells have a definite nucleus comprising the cell's genetic material, while prokaryotic cells don't have a nucleus and have free floating genetic material.
Some other differences includes Eukaryotes have membrane bound organelles while prokaryotes do not have membrane bound organelle. Prokaryotes have comparably less DNA then eukaryotes cells. Eukaryotic chromosomes are linear while prokaryotic chromosomes are circular. Prokaryotic cells do not have a true nucleus or membrane-bound organelles, whereas eukaryotic cells contain both a nucleus and organelles enclosed by membranes. All plant cells and animal cells are eukaryotic in nature .
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