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Equipment and supplies can be sterilized using autoclaves, clean-in-place (CIP) and sterilization-in-place (SIP) methods, dry heat sterilizers and ovens, steam disinfecting, media disinfecting, and UV chambers.

  • Physical or mechanical, chemical, and biological monitoring must be used in conjunction to verify the efficacy and proper operation of a sterilizer.
  • The use of one indication type DOES NOT eliminate the requirement for the other two types.
  • Physical Techniques: Ultrasonic Technique: Radiation Technique
  • Chemical Approach
  • The most widely used method of verifying sterilization is biological markers, or spore tests because they offer all materials can be sterilized or disinfected with the use of heat treatment.
  • The majority of common aquatic infections may be effectively eliminated by boiling.
  • A variety of sterilization techniques are used to clean medical equipment, including steam, dry heat, radiation, ethylene oxide gas, evaporated hydrogen peroxide, and other sterilization techniques (for example, chlorine dioxide gas, vaporized peracetic acid, and nitrogen dioxide).

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