The Ohio River Valley was significant because it was the catalyst for the French and Indian War.
The French and the British both laid claims to the areas around the Ohio River Valley and when the French tried to enforce this claim, war broke out between them and the British in the French and Indian War.
This war then spread to Europe where it became the Seven Years War and spread across the world.
In conclusion, the Ohio River Valley was significant because it was the reason the French and Indian War broke out.
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