Respuesta :
Decellularized porcine saphenous artery for small diameter tissue engineered conduit grafts.
Overview:
Decellularized xenografts have been identified as potential scaffolds for small-diameter vascular grafts. The purpose of this study was to biomechanically transform the porcine decellularized saphenous artery (DPSA) by a modified decellularization process using a Triton X-100/NHOH solution and serum-containing medium.
The goal was to develop and explore functional, biocompatible acellular conduits. Histological and biochemical analyzes showed a high degree of cell clearance and extracellular matrix preservation. Burst pressure tests showed that DPSA can withstand pressures of 1854 ± 164 mm Hg.
In vitro cell adhesion and biocompatibility evaluations showed that porcine pulmonary artery endothelial cells can adhere and grow on DPSA in static and rotary cultures. After implantation into the rabbit carotid artery in vivo, DPSA showed a patency rate of 60% at 1 month and 50% at 3 months.
No aneurysm and intimal hyperplasia were observed with any of his DPSA. All patented grafts showed regeneration of vascular elements and thrombotic occlusion was found to be the major cause of graft failure.
This is probably due to residual xenoantigens. In summary, this study demonstrated the development and evaluation of a decellularization process that could be used as a small-diameter graft.
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