1.67 mM is the substrate concentration when this enzyme operates at one‑quarter of its maximum rate.
It is given that the km of enzyme is 5.0 mM.
Enzyme is a protein based molecule that increase the rate of the given chemical reaction.
The conncetion in the middle of substrate and rate of reaction is calculated by the Michaelis-Menten Equation.
V = Vmax[S] / Km + [S]
where, V is initial velocity Km is measure of affinity and S is substrate concentration.
V = 0.25 [5.0] / 1/4 [5.0]
V =1.67
We apply one quarter in the given equation and the answer would be 1.67 mM
To know more about Michaelis-Menten Equation here :
https://brainly.com/question/20912949?referrer=searchResults
#SPJ4
