Thick or dense smears less likely to provide a good smear preparation for microscopic because it will diminish the amount of light that can pass through making it difficult to visualize the morphology of single cells under the microscope. Some times the stain can't penetrate all of the bacteria.
What is a microscopic smear?
- Smear is a specimen for microscopic study.
- The sample to be examined like blood, microbial culture etc. being spread thinly and unevenly across the slide with a swab or loop, or with the edge of another slide.
- The technique of smear preparation is that spreading small volume of sample on a slide and air drying the film before staining and microscopy.
- There are four types of smearing methods they are conventional method, blood film method, drop and rest method, and water-wash method.
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