Respuesta :
A TRAMP complex, which affixes a short poly(A) terminal to RNA destined for decay, is required for the effective turnover of various poorly digested RNAs in yeast, notably hypomodified tRNAi Met.
The poly(A) tail promotes exosome breakdown.
What is Degradation of hypomodified tRNAi?
Trf4p, a RNA-binding proteins Air2p, as well as the DExH RNA helicase Mtr4p are all members of the TRAMP complex. The function of Mtr4p in TRAMP-mediated RNA degradation pathways has remained unknown.
Some key features regarding the study of degradation of the hypomodified tRNAi are-
- It was shown that MTR4 is essential for degradation but not polyadenylation for hypomodified tRNAi Met using a genetic study.
- MTR4 was found to be a suppressor of a trm6-504 mutation with in tRNA m1A58 methyltransferase (Trm6p/Trm61p), that results in a lower level of tRNAi Met.
- This mtr4-20 mutation altered a single amino acid in Mtr4p's conserved helicase motif VI.
- In vivo, this mutation stabilizes hypomodified tRNAi Met but has no influence on TRAMP complex durability or polyadenylation activity.
- Purified recombinant Mtr4p also exhibits RNA-dependent ATPase activity that unwinds RNA duplexes with such a 3′-to-5′ polarity inside an ATP-dependent manner.
- The recombinant mutant Mtr4-20p has significantly reduced unwinding & RNA-stimulated ATPase activity, indicating that all these activities of Mtr4p are required for the degradation with polyadenylated hypomodified tRNAi Met.
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