Lysosomes, transport vesicles, plasma membrane, and nuclear membranes would be also affected by the use of Brefeldin A.
Brefeldin A's effects on endosomes, lysosomes, and the trans-Golgi network (TGN) suggest a general mechanism for regulating organelle structure and membrane traffic.
Brefeldin A (BFA) is a reversible protein transport inhibitor commonly used to enhance intracellular cytokine staining signals by blocking transport processes during cell activation.
The drug causes a reversible disruption of the Golgi by releasing the vesicle coat proteins and blocking the assembly of vesicles destined to move to the next compartment.
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