For the exploitation and manufacturing of functional milk, it is crucial to choose raw milk with high amounts of functional components that have health-promoting properties, but research on the variations in the functional components of whey from buffalo grown at various elevations is lacking.
DIA proteomics
- Here, we used data-independent acquisition proteomic techniques to identify the impacts of altitudes on the functional components in whey from dairy buffalo farms located at low altitude (LA), medium altitude (MA), and high altitude (HA) sites.
- 33 samples had 9331 peptides that corresponded to 1008 high-confidence proteins. Compared to LA- and MA-whey, HA-whey had lower levels of angiotensinogen, but on the other hand, it had larger concentrations of immune-stimulating ingredients.
- Different proteins were engaged in the secretion, synthesis, and regulation pathways in immunological components, complement and coagulation cascades, and vascular smooth muscle contraction.
- In comparison to HA-whey, LA-whey had higher concentrations of seleno protein F and lymphocyte antigen.
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