For maximum efficiency in loading oxygen at the lungs, the temperature should be slightly lower than normal body temperature.
A student in your lab volunteers to enter a hypoxic breathing chamber for 10 minutes, and his alveolar PO2 drops to 50 mm Hg.
Blood pH: The hydrogen ion concentration [H +] influences the affinity of Hb for O2.
Increases in [H +] (decrease pH) decrease affinity and shift the curve rightwards, a phenomenon known as the Bohr shift.
This promotes unloading of O2, but does not affect loading (because the curve is flat in this region).
The opposite explains: the higher the pH, the lower the H ion concentration, the lower the carbon dioxide level, and the GREATER affinity hemoglobin has for oxygen.
The binding of oxygen to hemoglobin in the lungs is not affected by changing the pH and the oxygen will continue to be loaded normally.
learn more about pH here: