A student is looking at a bacterial specimen using the oil immersion lens, but has forgotten to put immersion oil on the slide. The specimen will appear somewhat fuzzy and have poor resolution.
- Oil immersion is a method for boosting a microscope's resolving ability in light microscopy.
- This is accomplished by raising the numerical aperture of the objective lens by submerging the specimen and the objective lens in a transparent oil with a high refractive index.
- By substituting a high refractive index medium for the air gap between the immersion objective lens and cover glass and lowering light refraction, immersion oil improves the microscope's ability to resolve small details.
- More light is directed through the objective and a clearer image is seen by inserting a material, such as immersion oil, with a refractive index equivalent to that of the glass slide in the space filled with air.
- Thus, the specimen will appear somewhat fuzzy and have poor resolution.
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