Respuesta :
These probes are likely to be of considerable value to the wheat community.
How can sequence-based single nucleotide polymorphic markers be adapted for targeted genotyping by sequencing in hexaploid wheat?
- Both wheat breeders and researchers use single nucleotide polymorphisms (SNPs) as molecular markers to identify regions of interest within the hexaploid wheat genome. Several SNP-based genotyping systems are available, and their usefulness depends on various elements, including the technology available, the quantity of data necessary, the budget, and the level of technical competence needed.
- Unfortunately, the exchange of markers across current and newly developed platforms is rarely possible, making it impossible to compare or merge older data sets with more recent ones. Within the next five to ten years, genotyping by sequencing will likely overtake other genotyping techniques as the standard method.
- With this in mind, we built and used SNP-based capture probes from thousands of already-existing, publicly-available probes from the Axiom® and KASPTM genotyping platforms to ensure that data was collected from current genotyping platforms continues to be useful. In a targeted genotyping by sequencing technique, we have verified our capture probes using 31 previously genotyped UK elite hexaploid wheat accessions.
- A set of 3256 probes was discovered through data comparisons among targeted genotyping by sequencing, array genotyping, and KASPTM assays that successfully combine the data from targeted genotyping by sequencing with the set of markers that were previously accessible.
These probes are likely to be of considerable value to the wheat community.
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