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A new rainforest plant was discovered by scientists, having been used by native cultures for generations to cure fungal infections. Jane is a scientist in charge of investigating the anti-fungal properties of the new plant species. Describe the molecular techniques that Jane could use to isolate, identify, and produce the anti-fungal compound in the laboratory.

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Fungi detection using molecular methods

PCR as we know it. The invention of PCR ushered in a major shift in the field of plant pathology. PCR that is nested. To achieve a high level of specificity and sensitivity, nested PCR is used. Multiplex PCR.

RT-PCR

Fungi were isolated from the root samples that were obtained. After removing soil debris with tap water, samples were submerged in 1 percent sodium hypochlorite NaOCl) for 1-2 minutes. After 2-3 minutes in sterile distilled water (SDW), the samples were dried on sterile filter paper.

When identifying, look for the following essential characteristics:

  • Shape, color, and size of the fruiting body

  • A spore print can also be acquired from the gills, specifically how they adhere to the stem.

  • Shape, color, and size of the stem

  • The smell and the texture.

Preformed antifungal chemicals found naturally in healthy plants are thought to act as intrinsic chemical barriers to infection, protecting plants from a wide spectrum of possible pathogens.

Learn more about fungus here: https://brainly.com/question/26298859

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