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Patch clamping technique we would use to study the effects of the extracellular environment on ion channel activity.
Patch clamp:
The patch clamp method in electrophysiology is a laboratory technique for examining ionic channels in isolated living cells, tissue samples, or patches of cell membrane. The method can also be used to examine bacterial ion channels in specially made gigantic spheroplasts. It is particularly helpful in the research of excitable cells such neurons, cardiomyocytes, muscle fibers, and pancreatic beta cells.
The voltage clamp method can be used to perform patch clamping. In this instance, the experimenter controls the voltage across the cell membrane, and the ensuing currents are measured. As an alternative, the current clamp method can be applied. In this instance, the experimenter controls the current that crosses the membrane, and the ensuing variations in voltage are recorded, usually in the form of action potentials.
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