Refrigeration will prevent bacterial growth which may result in uricase production and breakdown of uric acid in the sample.
An enzyme called uricase is responsible for catalyzing the change of uric acid into allantoin. Your body excretes uric acid, an acidic waste product, through urine. It is a typical result of the digestion of purine-rich meals.
Only media containing uric acid, allantoin, or allantoate as the primary or only source of carbon, nitrogen, and energy support the growth of the bacteria Bacillus fastidious. Raw extract of cells cultivated on uric acid had significant amounts of uricase.
Alcaligenes eutrophus, Pseudomonas aeruginosa, and Micrococcus denitrificans all displayed increased uricase activity after being placed in conditions containing uric acid, according to Kaltwasser. The ability of microorganisms to degrade uric acid and to use it for growth is an inducible property of various bacteria. Uricases of Bacillus fastidious and of Aspergillus nidulans were reported to be induced by uric acid.
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