Frozen red blood cells that have been thawed, deglycerolized and reconstituted in an open system must be used within _______.

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13-07-2022
Biology

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Frozen red blood cells that have been thawed, deglycerolized and reconstituted in an open system must be used within _______.

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lalitasinghvaidikedu lalitasinghvaidikedu · Answer 1 · 2022-07-20T13:41:22+02:00

Frozen red blood cells that have been thawed, deglycerolized and reconstituted in an open system must be used within 24 hours.

Cryopreservation of Red blood cells (RBCs) can be done with a long time span of usability of 10 years. Glycerol safeguards RBCs during freezing and defrosting, yet it can cause hemolysis if it is not washed off or eliminated before transfusion. When a unit has been deglycerolized, the shelf life of RBCs in an open system is only 24 hours, bringing about sporadic utilization of Frozen RBCs from the outset of the procedure or in close system cases it is up to 14 days.

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shagunchoudhary shagunchoudhary · Answer 2 · 2022-07-20T13:43:57+02:00

Frozen red blood cells that have been thawed, deglycerolized and reconstituted in an open system must be used within 24 hours.

The classical way of preserving the blood cells is to store them at low temperatures. The phenomenon by which the red blood cells are frozen and defrosted is called cryopreservation. This process requires the use of glycerol to safeguard the RBCs from damage. The glycerol is washed off prior to transfusion to avoid hemolysis. After removal of glycerol, the shelf life of RBCs in case of an open system is only for 24 hours. This creates the need of immediate use of the frozen RBCs. However, in case of close system, the RBC utilization period is up to 14 days.

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