Respuesta :
Nucleases are a class of hydrolases that divide the phosphodiester bonds between the nucleotides in the nucleic acids, both DNA and RNA. The significant job of nucleases inside the cell is in the DNA repair systems.
The major distinction between endonucleases and exonucleases is that endonuclease divides nucleic acid strands at the center which brings about the making of oligonucleotides with the formation of blunt or sticky ends while exonuclease severs nucleic acids strands from the ends which results in the creation of single nucleotides or nucleosides with the formation of sticky ends.
Exonucleases can go about as editors or more scientifically termed proofreaders during DNA polymerization in DNA replication, to eliminate uncommon DNA structures that can emerge any issues with DNA replication fork movement, and can be associated with repairing damaged DNA.
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Exonucleases are the DNA cutting enzymes that act only on DNA ends. Endonucleases on the other hand cut the DNA molecule in between the nucleotide sequence.
Exonucleases play important role in homologous recombination repair by acting as proofreading and editing machinery during the time of DNA synthesis. These enzymes remove the damaged DNA nucleotides from the ends of the DNA or DNA fragment to eliminate the chances of mutations in the DNA. This helps in maintaining DNA integrity.
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