When selecting a photometric technique that is more sensitive to a low concentration of analyte, a laboratory director compares absorbance measuring devices against those that measure fluorescence intensity. Fluorescence spectroscopy is considered more sensitive in its measurements.
Spectro-fluorometer works on the above mentioned principle...
- It uses a beam of light to excite the electrons from the given material which cause the emission of light from that matter.
- The emitted light is then brought towards a filter and onto a detector for measurement and identification of the changes in molecules of a sample material.
- Fluorometry is sensitive technique because light of particular wavelength is required as electrons are getting excited from ground state to emit the light and shows particular results which helps to know the sample material.
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