Thin layer chromatography(TLC) works with the principle of separation through adsorption.
It is used in the isolation and extraction of lipids through the following steps:
- apply the lipid samples spots in the bottom of the plate.
- also apply sample solution to the marked spot
- pour the mobile phase into the TLC chamber and use a moist filter paper to cover it. this is done to maintain equal humidity.
- then place the plate in the TLC chamber and close it with a lid.
- the plate is immersed into the solvent (mobile phase) for its development. this is done, keeping in mind that the sample spot should be above the solvent.
- once the sample spots are developed, they are removed and dried.
- this is later viewed using the UV light chamber to see the isolation of the lipid sample.
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