The statement which is not true of the purpose of the alcohol in Gram staining is as follows:
- It binds the crystal violet into the cell wall.
Thus, the correct option is A.
What is Gram staining?
Gram staining may be defined as a methodology that is utilized for the classification of bacterial species such as gram-positive and gram-negative.
In gram staining, ethyl alcohol or acetone is used as a decolorizer that dehydrates the peptidoglycan layer. It involves the shrinkage or tightening of the cell wall.
In this process, the large crystal violet-iodine complex is not able to puncture this tightened layer of peptidoglycan.
Therefore, the correct option for this question is A.
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