Suppose a solution was too concentrated for an accurate reading with the spectrophotometer. The concentrated solution was diluted by placing 1.00 mL of the concentrated solution in 4.00 mL of water. The solution was then placed in the spectrophotometer and an absorbance was obtained and after a few calculations the molar concentration was calculated to be 3.5 × 10–6 M. what was the concentraion of the original solution

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Answer:

14µM

Explanation:

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[tex]1.75\times 10^{-5} M[/tex] was the concentration of the original solution.

Explanation:

  • The equation of dilution is given as:

        [tex]M_1V_1=M_2V_2[/tex]

Where:

     [tex]M_1[/tex]=The concentration of the solution before dilution

      [tex]V_1[/tex]= Volume of solution before dilution

      [tex]M_2[/tex]=The concentration of the solution after dilution

      [tex]V_2[/tex]= Volume of solution after dilution

Given:

The 1.00 mL of solution was diluted with 4.00 mL of water.

The concentration of a diluted solution is [tex]3.5\times 10^{-6 M}[/tex].

To find:

The original concentration of the solution before dilution.

Solution:

The original concentration of solution before dilution = [tex]M_1[/tex]

The volume of original solution used = [tex]V_1 = 1.00 mL[/tex]

The volume of water added for dilution = v = 4.00 mL

The concentration of solution after dilution = [tex]M_2=3.5\times 10^{-6 }M[/tex]

The volume of diluted solution used = [tex]V_2[/tex]

[tex]V_2= V_1+v=1.00 mL+4.00 ml=5.00 mL[/tex]

Using the equation of dilution:

[tex]M_1V_1=M_2V_2\\M_1=\frac{M_2V_2}{V_1}\\M_1=\frac{3.5\times 10^{-6 M}\times 5.00 mL}{1.00 mL}\\M_1=1.75\times 10^{-5}M[/tex]

[tex]1.75\times 10^{-5} M[/tex] was the concentration of the original solution.

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