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Bob uses a scalpel to get a thin slice of potato for observation under a microscope. He prepares a wet mount of the specimen and then views it under the compound light microscope. He can barely see the cells of the potato. What can he do in terms of specimen preparation to improve what he is seeing? (Note: Do not discuss controls on the microscope that require adjusting.)

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marianaegarciaperedo

Answer:

Bob can use Lugol to dye or stain the potato tissue and get to see the cells.

Explanation:

After viewing the slice of potato under the microscope, Bob can perform dyeing using Lugol to improve the vision and get to differentiate the cells.

All he needs to do is to add a few drops of Lugol between the glass slide and the coverslip, without taking this last one out. He needs to cover the whole potato slice with Lugol.

Once the tissue is covered with Lugol, Bob can eliminate the excess of the liquid, and then view the slide again under the microscope.

  • At 4X, Bob will see little violet spheric structures that are conglomerated.
  • At 10X and 40X, Bob will be able to discriminate each dyed cell. At this point, he will be able to see the nucleus, cytoplasm, nuclear membrane, and cellular wall. He will also see amyloplasts (the place where the cell storage starch) in the interior of the cells.

 

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