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PLEASE HELP -- Imagine protein X, destined to span the plasma membrane. Assume that the mRNA carrying the genetic message for protein X has already been translated by ribosomes in a cell culture. If you fractionate the cells, in which fraction would you find protein X? Explain by describing its transit through the cell.

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luisvaschetto

Answer:

Protein X would be ultimately present in the subcellular fraction containing membrane fragments

Explanation:

It is possible to trace the synthesis and transport of a membrane protein by the following steps:

1- The amino acids of the membrane protein are joined together in the ribosome to form a polypeptide chain (in this case, the protein X).

2- A signal sequence of the growing polypeptide produces the binding of the ribosome to the membrane of the endoplasmic reticulum (ER) and the insertion of this polypeptide chain into the ER.

3-The protein is released into a vesicle from the ER to be exported to the Golgi complex.

4- The membrane protein is transported by the Golgi complex to the cell membrane by the formation of a secretory vesicle that fuses with the plasma membrane, thus being finally embedded in the lipid bilayer.

Protein X should be present in the subcellular fraction that comprise of membrane fragments

The fraction that should be measured the protein X:

Here the synthesis should be traced and the membrane protein transport should be applied by using the following steps:

  • Here the amino acids of the membrane protein should be connected together so that the polypeptide chain should be created.
  • A signal sequence with respect to the polypeptide should be grown that generated the ribosome binding.
  • Now the protein should be relased into the vesicle.
  • At last, the protein of the membrane should be transported to the cell membrane.

Learn more about cell here: https://brainly.com/question/13250146

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