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Each of the following statements about the citric acid cycle is true except:_________. A. Decarboxylation to release CO2 occurs at two steps. B. The cycle involves the ordered activity of eight enzymes. C. Substrate-level phosphorylation generates ATP/GTP at one step. D. Oxidation by transfer of electrons to coenzymes occurs at two steps. E. Acetate enters the citric acid cycle and joins a four-carbon acceptor molecule to form citrate.

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Answer: E). Acetate enters the citric acid cycle and joins a four-carbon acceptor molecule to form citrate.

Explanation: Acetyl CoA enters the citric acid cycle and combines with oxaloacete (a four carbon acceptor molecule) to form citrate. Citric acid cycle is a cycle of eight enzyme catalyzed reactions in which acetyl CoA from pyruvate is oxidized to generate ATP/GTP, NADH and FADH2. Decarboxylation occurs in citric acid cycle at two steps catalyzed by isocitrate dehydrogenase and alpha ketoglutarate dehydrogenase complex. Substrate level phosphorylation occurs at the step catalyzed by succinyl CoA synthase in which GTP is produced from addition of a phosphoryl group to ADP.

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