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Suppose that there are individuals in the population that have a mutation in an enzyme gene. The residue at position 50, which is normally aspartate, is substituted with alanine (D50A). The D50A mutation occurs at an area within the active site that is important for substrate binding, but not for enzyme catalysis.

(a) Predict how this would affect the KM, Vmax, and kcat for the enzyme. Insert your answer
(b) Compared to what you learned about inhibitors is this case, what type of inhibition would this mutation resemble?

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Answer:

a) Km = increase, Vmax and kcat = remain same

b) resembles competitive inhibition

Explanation:

Given Data:

  • presence of individuals in population having mutation in enzyme gene.
  • residue at position 50 substituted with alanine.
  • location : site important for substrate bonding but not for enzyme catalysis.

a)

There would be increase in value of Km.

As higher substrate presence could overcome the presence of inhibitors so Vmax value would remain same.

The value of Kcat would also remain the same.

b)

The type of inhibition this mutation resembles is that of competitive inhibition.

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