The molar extinction coefficient of cytochrome P450, one of the compounds involved in photosynthesis, at 522 nm is 291 L mol-1cm-1. When light of that wavelength passes through a cell of length 6.5 mm containing a solution of P450, 39.8 % of the light was absorbed. What is the molar concentration of the solution?

Respuesta :

Answer:

0.001165 mol/L is the molar concentration of the solution.

Explanation:

Using Beer-Lambert's law :

Formula used :

[tex]A=\epsilon \times C\times l[/tex]

[tex]A=\log \frac{I_o}{I}[/tex]

[tex]T=\frac{I}{I_o}[/tex]

[tex]\log \frac{I_o}{I}=\epsilon \times C\times l[/tex]

where,

A = absorbance of solution

C = concentration of solution

l = path length

T = Transmittance

[tex]I_o[/tex] = incident light

[tex]I[/tex] = transmitted light

[tex]\epsilon[/tex] = molar absorptivity coefficient

We have :

Transmittance of the light  = 100% - 39.8 % = 60.2 % = 0.602

Absorbance of the light :

[tex]A=\log \frac{1}{T}=\log \frac{1}{0.602}=0.2204[/tex]

Molar absorptivity coefficient  = [tex]\epsilon = 291 L mol^{-1} cm^{-1}[/tex]

Length of the cell = l  6.5 mm = 0.65 cm ( 1 mm = 0.1 cm)

Now put all the given values in the above formula, we get the concentration of solution :

[tex]0.2204=291 L mol^{-1} cm^{-1}\times (C)\times (0.65 cm)[/tex]

[tex]C=0.001165 mol/L[/tex]

0.001165 mol/L is the molar concentration of the solution.