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Answer:
The correct option is A.
Explanation:
Various complex organisms have their nucleus enclosed by a membrane which is termed as the nuclear membrane. The nuclear membrane separates the material inside the nucleus from rest of the cell. Simple organisms like prokaryotes lack a membrane-bound nucleus. Their hereditary material is dispersed into the cytoplasm without any nuclear membrane. Hence, two-kingdom classification can be based on this feature.
All other options are wrong because plasma membrane, cytoplasm and genetic material is present in every cell. Hence, organisms cannot be differentiated based on them.