Select the correct characteristics describing normal-phase and reversed-phase chromatography. Normal-phase chromatography Reversed-phase chromatography (a) Polarity of phases: 1. Stationary phase is polar 2. Stationary phase is non polar b) Eluent strength of solvent. 1. Increases as solvent becomes more polar. 2. Increases as solvent becomes less polar c) Nature of solutes. 1. Polar 2. Non-polar d) Nature of solute interaction. 1. More soluble in mobile phase as the polarity of the mobile phase increases. 2. More soluble in mobile phase as the polarity of the mobile phase decreases. e) Polarity of phases: 1. Stationary phase is polar 2. Stationary phase is non-polar f) Eluent strength of solvent: 1. Increases as solvent becomes more polar. 2. Increases as solvent becomes less polar. g) Nature of solutes: 1. Polar 2. Non-Polar h) Nature of solute interaction: 1. More soluble in mobile phase as the polarity of the mobile phase increases. 2. More soluble in mobile phase as the polarity of the mobile

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Answer:

(a) Polarity of phase: 1. Stationary phase is polar

b) Eluent strength of solvent:  1. Increases as solvent becomes more polar

c) Nature of solutes.1. Polar

d) Nature of solute interaction:2. More soluble in mobile phase as the polarity of the mobile phase decreases

e) Polarity of phases: 1. Stationary phase is polar

f) Eluent strength of solvent: 1. Increases as solvent becomes more polar.

g) Nature of solutes: 1. Polar

h) Nature of solute interaction: 2. More soluble in mobile phase as the polarity of the mobile

Explanation: