Respuesta :
The correct answer is A. Using the same restriction enzyme for cutting the desired gene and the vector and using ligase for pasting the gene into the vector. Vectors ensure DNA replication in cells. Restriction enzymes slice the DNA in specific sites; it should be the same so as the two ends will easily complement back together.
This is carried out using the same restriction enzyme for cutting the desired gene and the vector and using ligase for pasting the gene into the vector. The correct option among all the options that are given in the question is the first option or option "A". I hope that this is the answer you were looking for and it has helped you.